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rabbit anti sestrin2  (Proteintech)


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    Structured Review

    Proteintech rabbit anti sestrin2
    Rabbit Anti Sestrin2, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+sestrin2+antibody/Sestrin+2+Polyclonal+antibody/pmc13028361-97-13-16
    Average 95 stars, based on 155 article reviews
    rabbit anti sestrin2 - by Bioz Stars, 2026-09
    95/100 stars

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    Centrifugation:

    Article Title: Modulators of Sestrin-GATOR2 interaction and uses thereof
    Article Snippet: After compound incubation, samples were subjected to immunoprecipitation with the CST anti-Sestrin2 antibody for 1.5 hours followed by a 30-minute incubation with protein-A sepharose as described in L. Chantranupong, et al., Cell Reports 9:1-8 (2014). .. The sepharose conjugated antibody-protein complex was precipitated via centrifugation and the flow-through was subjected to a second round of immunoprecipitation with a rabbit polyclonal anti-Sestrin2 antibody (Protein Tech, #10795-1-AP) to determine that total Sestrin2 protein levels between samples were equal. .. SDS-PAGE was performed with the immunoprecipitation samples followed by western-blot with the mouse monoclonal anti-Sestrin2 antibody from SIGMA (cat #WH0083667M3).

    Article Title: Modulators of sestrin-GATOR2 interaction and uses thereof
    Article Snippet: After compound incubation, samples were subjected to immunoprecipitation with the CST anti-Sestrin2 antibody for 1.5 hours followed by a 30-minute incubation with protein-A sepharose as described in L. Chantranupong, et al., Cell Reports 9:1-8 (2014). .. The sepharose conjugated antibody-protein complex was precipitated via centrifugation and the flow-through was subjected to a second round of immunoprecipitation with a rabbit polyclonal anti-Sestrin2 antibody (Protein Tech, #10795-1-AP) to determine that total Sestrin2 protein levels between samples were equal. .. SDS-PAGE was performed with the immunoprecipitation samples followed by western-blot with the mouse monoclonal anti-Sestrin2 antibody from SIGMA (cat #WH0083667M3).

    Immunoprecipitation:

    Article Title: Modulators of Sestrin-GATOR2 interaction and uses thereof
    Article Snippet: After compound incubation, samples were subjected to immunoprecipitation with the CST anti-Sestrin2 antibody for 1.5 hours followed by a 30-minute incubation with protein-A sepharose as described in L. Chantranupong, et al., Cell Reports 9:1-8 (2014). .. The sepharose conjugated antibody-protein complex was precipitated via centrifugation and the flow-through was subjected to a second round of immunoprecipitation with a rabbit polyclonal anti-Sestrin2 antibody (Protein Tech, #10795-1-AP) to determine that total Sestrin2 protein levels between samples were equal. .. SDS-PAGE was performed with the immunoprecipitation samples followed by western-blot with the mouse monoclonal anti-Sestrin2 antibody from SIGMA (cat #WH0083667M3).

    Article Title: Modulators of sestrin-GATOR2 interaction and uses thereof
    Article Snippet: After compound incubation, samples were subjected to immunoprecipitation with the CST anti-Sestrin2 antibody for 1.5 hours followed by a 30-minute incubation with protein-A sepharose as described in L. Chantranupong, et al., Cell Reports 9:1-8 (2014). .. The sepharose conjugated antibody-protein complex was precipitated via centrifugation and the flow-through was subjected to a second round of immunoprecipitation with a rabbit polyclonal anti-Sestrin2 antibody (Protein Tech, #10795-1-AP) to determine that total Sestrin2 protein levels between samples were equal. .. SDS-PAGE was performed with the immunoprecipitation samples followed by western-blot with the mouse monoclonal anti-Sestrin2 antibody from SIGMA (cat #WH0083667M3).

    other:

    Article Title: Modulators of sestrin-GATOR2 interaction and uses thereof
    Article Snippet: After compound incubation, samples were subjected to immunoprecipitation with the CST anti-Sestrin2 antibody for 1.5 hours followed by a 30-minute incubation with protein-A sepharose as described in L. Chantranupong, et al., Cell Reports 9:1-8 (2014).

    Article Title: Modulators of sestrin-GATOR2 interaction and uses thereof
    Article Snippet: After compound incubation, samples were subjected to immunoprecipitation with the CST anti-Sestrin2 antibody for 1.5 hours followed by a 30-minute incubation with protein-A sepharose as described in L. Chantranupong, et al., Cell Reports 9:1-8 (2014).

    Article Title: Modulators of Sestrin-GATOR2 interaction and uses thereof
    Article Snippet: After compound incubation, samples were subjected to immunoprecipitation with the CST anti-Sestrin2 antibody for 1.5 hours followed by a 30-minute incubation with protein-A sepharose as described in L. Chantranupong, et al., Cell Reports 9:1-8 (2014).

    Article Title: Modulators of Sestrin-GATOR2 interaction and uses thereof
    Article Snippet: After compound incubation, samples were subjected to immunoprecipitation with the CST anti-Sestrin2 antibody for 1.5 hours followed by a 30-minute incubation with protein-A sepharose as described in L. Chantranupong, et al., Cell Reports 9:1-8 (2014).

    Article Title: Modulators of Sestrin-GATOR2 interaction and uses thereof
    Article Snippet: After compound incubation, samples were subjected to immunoprecipitation with the CST anti-Sestrin2 antibody for 1.5 hours followed by a 30-minute incubation with protein-A sepharose as described in L. Chantranupong, et al., Cell Reports 9:1-8 (2014).



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    KEY RESOURCES TABLE

    Journal: Molecular cell

    Article Title: Lineage-restricted regulation of SCD and fatty acid saturation by MITF controls melanoma phenotypic plasticity

    doi: 10.1016/j.molcel.2019.10.014

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rabbit monoclonal anti-SESTRIN2 , Protein Tech , Cat#10795–1-AP.

    Techniques: Isolation, Virus, Subcloning, Bacteria, Recombinant, Multiplex sample analysis, Labeling, Reverse Transcription, RNA Extraction, Reporter Assay, Bicinchoninic Acid Protein Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Control, Plasmid Preparation, Expressing, Construct, Luciferase, Software